Browsing by Author "Chrisp, CE"

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    Protection of rabbits against experimental pasteurellosis by vaccination with a potassium thiocyanate extract of Pasteurella multocida.
    (Mount Kenya University, 1985-09) Ringler, DH; Peter, GK; Chrisp, CE; Keren, DF
    Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida serotype 2 weeks after the second vaccination. The vaccinated rabbits produced serum immunoglobulin G and nasal mucosal immunoglobulin A against P. multocida. The vaccine protected the challenged rabbits against clinical disease and death; however, otitis media was not prevented, and microscopic inflammatory lesions were occasionally noted in the lungs and nasal turbinates. In contrast, nonvaccinated, challenged rabbits became febrile, dyspnic, depressed, and anorectic, and five of six died within 4 days of challenge with severe lesions including pneumonia, pleuritis, otitis media, and bacteremia. The vaccine prevented death and colonization of challenge organisms in the blood and lung, but did not prevent colonization of the middle ear. The vaccine alone did not cause clinical disease or gross lesions, but did produce microscopic pulmonary inflammatory lesions.
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    PublicationOpen Access
    Serological analysis of five serotypes of Pasteurella multocida of rabbit origin by use of an enzyme-linked immunosorbent assay with lipopolysaccharide as antigen.
    (Mount Kenya University, 1984-08) Cary, CJ; Peter, GK; Chrisp, CE; Keren, DF
    The serological relationships among five Pasteurella multocida strains, representing five somatic serotypes most commonly isolated from rabbits (serotypes 1, 3, 4, 12, and 15), were studied with an enzyme-linked immunosorbent assay. Lipopolysaccharides from the five serotypes were used as antigens in the assay. Antisera against serotypes 1 and 15 reacted only with their homologous lipopolysaccharides. Significant cross-reactivity was found between serotypes 4 and 12. Serotype 3 antisera showed minimal reactivity with both homologous and heterologous lipopolysaccharides. The feasibility of detecting antibodies to each of several lipopolysaccharide antigens combined in the same assay cell well was demonstrated.
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    PublicationOpen Access
    Spontaneous hyperthyroidism in an aged male and female Macaca mulatta.
    (Mount Kenya University, 1998-12-27) Brammer, DW; Juneau, PL; Chrisp, CE; O'Rourke, CM; Altrogge, DM; Peter, Gk; Hofing, Gl
    A 31-year-old male and a 31-year-old female rhesus monkey developed clinical signs consistent with hyperthryoidism. These included a ravenous appetite, hyperactivity, and accentuated ratchet movement and hand tremors while performing fine motor tasks. Bilaterally enlarged thyroid glands were palpated in both monkeys. A unique clinical finding of the female as the hypertrophic cardiomyopathy. The T3 and T4 levels in the male rhesus were 3.79 ng/ml and 28.20 microg/dl, respectively. T3 and T4 levels in the female were 4.33 ng/ml and 22.2 microg/dl, respectively. A biopsy of the enlarged thyroids demonstrated a typical multinodular goiter with cystic hyperplasia. The female rhesus was successfully treated with methimazole, but the hypertrophic cardiomyopathy did not resolve. The relationship between erythrocytosis and T4 levels common to humans and cats is also evident in the rhesus monkey.