Browsing by Author "Keren, DF"

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    Protection of rabbits against experimental pasteurellosis by vaccination with a potassium thiocyanate extract of Pasteurella multocida.
    (Mount Kenya University, 1985-09) Ringler, DH; Peter, GK; Chrisp, CE; Keren, DF
    Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida serotype 2 weeks after the second vaccination. The vaccinated rabbits produced serum immunoglobulin G and nasal mucosal immunoglobulin A against P. multocida. The vaccine protected the challenged rabbits against clinical disease and death; however, otitis media was not prevented, and microscopic inflammatory lesions were occasionally noted in the lungs and nasal turbinates. In contrast, nonvaccinated, challenged rabbits became febrile, dyspnic, depressed, and anorectic, and five of six died within 4 days of challenge with severe lesions including pneumonia, pleuritis, otitis media, and bacteremia. The vaccine prevented death and colonization of challenge organisms in the blood and lung, but did not prevent colonization of the middle ear. The vaccine alone did not cause clinical disease or gross lesions, but did produce microscopic pulmonary inflammatory lesions.
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    PublicationOpen Access
    Serological analysis of five serotypes of Pasteurella multocida of rabbit origin by use of an enzyme-linked immunosorbent assay with lipopolysaccharide as antigen.
    (Mount Kenya University, 1984-08) Cary, CJ; Peter, GK; Chrisp, CE; Keren, DF
    The serological relationships among five Pasteurella multocida strains, representing five somatic serotypes most commonly isolated from rabbits (serotypes 1, 3, 4, 12, and 15), were studied with an enzyme-linked immunosorbent assay. Lipopolysaccharides from the five serotypes were used as antigens in the assay. Antisera against serotypes 1 and 15 reacted only with their homologous lipopolysaccharides. Significant cross-reactivity was found between serotypes 4 and 12. Serotype 3 antisera showed minimal reactivity with both homologous and heterologous lipopolysaccharides. The feasibility of detecting antibodies to each of several lipopolysaccharide antigens combined in the same assay cell well was demonstrated.