Browsing by Author "Onyuka, Jackson Odhiambo"

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    PublicationOpen Access
    Antimicrobial Efficacy Study Of Acmella Caulirhiza And Spermacoce Princeae Used By Postpartum Mothers In Nyamira County, Kenya
    (International Journal of Scientific and Research Publications, 2021-11) Onyango, Jespher Nyaboke; Onyuka, Jackson Odhiambo; Onyancha, Jared Misonge; Getonto, Patrick Ogembo
    uerperal sepsis is the major cause of (31%) maternal mortality. Appropriate care could prevent majority of these deaths. Exogenous and endogenous organisms are the causatives agents. Traditional medicine have been used in treating postpartum sepsis for example Acmella caulirhiza used to treat a child’s mouth sores and Spermacoce princeae used to accelerate healing of umbilical cord and to clean the system after birth. The main objective of the present study was to determine antimicrobial activity levels of A. caulirhiza and S. princeae used by postpartum mothers in Nyamira County, Kenya. The study area was Nyamira County where the two plant specimen and clinical specimens of 339 mothers were collected. Plant materials were identified at East Africa Herbarium. Plant specimens were transported to M.K.U. Pharmacognosy laboratory where processing was done. Clinical specimens were collected from mothers at Nyamira Level 5 Hospital. A single high vaginal swab was collected from each participant, immediately inserted in stuart’s transport medium, labeled, placed in a cooler box, triple packaged and transported to Microbiology laboratory of M.K.U. within 48 hours where processing were done. Culture, isolation, identification were done. Disc diffusion method was employed to determine antimicrobial activity in the compounds of the crude plant extracts. Data was stored in Excel spread sheet in a personal computer protected with a password. Antimicrobial activity was analyzed using descriptive statistics. Data was presented using, tables and photographs. Results: Antibacterial activity of A. caurhiza and S. princeae indicated that, the whole plant extracts had low antibacterial effects against S. aureus and E. coli. No antifungal effects were detected for crude extracts of the two plants. Discussion; the two plants used by the local inhabitants of Nyamira County in treating postpartum infections indicated that, they possess low antibacterial activity against S. aureus and E. coli. However, no antifungal activity was detected from the two plants. Recommendation; the two plants may be used in treating postpartum infections though commercially available drugs are recommended as they are highly effective. The two plants indicated low antibacterial activity against S. aureus and E. coli. However, further studies are recommended on genotoxicity of this plant extracts on S. aureus and E. coli genes. Also further research is recommended to isolate and identify pure compounds of the two plants to establish pharmacological basis of antibacterial activity shown even though it was low
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    PublicationOpen Access
    Phytochemical Studies of Acmella Caulirhiza and Spermacoce Princeae used by postpartum mothers in Nyamira County, Kenya.
    (International Journal of Scientific and Research Publications, 2017-08) Onyango, Jespher Nyaboke; Onyancha, Jared Misonge; Onyuka, Jackson Odhiambo; Ochora, John Memba; Getonto, Patrick Ogembo; Maina, Charles Wambugu
    Abstract- Introduction: Traditional medicine have been used in health maintenance, disease prevention and treatment for example Acmella caulirhiza used to treat a child’s mouth sores and Spermacoce princeae used to accelerate healing of umbilical cord and to clean the system after birth. Objective: The main objective of the present study was to determine phytochemical compounds of A. caulirhiza and S. princeae used by postpartum mothers in Nyamira County, Kenya. Methodology: The study area was Nyamira County where the two plant specimens were collected. Plant materials were identified at East Africa Herbarium. Plant specimens were transported to M.K.U. Pharmacognosy laboratory where processing was done. Phytochemical analysis methods were employed to determine phytochemicals compounds in the crude plant extracts. Data was stored in Excel spread sheet in a personal computer protected with a password. Data was presented using tables and photographs. Results: Phytochemical examinations revealed that Acmella caulirhiza contains flavonoids, terpenoids, coumarins and sterols compounds. On the other hand, Spermacoce princeae contains flavonoids, terpenoids, tannins, saponin alkaloid and glycoside compounds. Conclusion and recommendation: The plants may be used in treating puerperal sepsis although commercially available drugs are recommended as they are highly effective. The two plants can be a potent source of complementary and modern medicine. Further research is recommended to isolate and identify pure compounds of the two plants
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    PublicationOpen Access
    Safety Studies of Acmella Caulirhiza And Spermacoce Princeae Used By Postpartum Mothers In Nyamira County, Kenya
    (International Journal of Scientific and Research Publications, 2022-09-15) Onyango, Jespher Nyaboke; Onyancha, Jared Misonge; Onyuka, Jackson Odhiambo; Ochora, John Memba; Getonto, Patrick Ogembo
    Introduction: Puerperal sepsis is the major cause of (31%) maternal mortality. Appropriate care could prevent majority of these deaths. Exogenous and endogenous organisms are the causatives agents. Traditional medicine have been used in treating postpartum sepsis for example Acmella caulirhiza used to treat a child’s mouth sores and Spermacoce princeae used to accelerate healing of umbilical cord and to clean the system after birth. Objestive: The main objective of the present study was to determine safety of A. caulirhiza and S. princeae used by postpartum mothers in Nyamira County, Kenya. Methodology: The study area was Nyamira County where the two plant specimens were collected. Plant materials were identified at East Africa Herbarium. Plant specimens were transported to Mount Kenya University Pharmacognosy laboratory where processing was done. Brine shrimp toxicity testing methods were employed to determine safety of the two crude plant extracts. Data was stored in Excel spread sheet in a personal computer protected with a password. Toxicity results were analyzed using the graph pad prism version 5 to estimate IC50 values. Data was presented using, tables and photographs. Results: Brine shrimp lethality test of the two plants indicated that the plants were non-toxic. Conclusion and recommendation: The two plants may be considered safe for use in treating puerperal sepsis though commercially available drugs are recommended as they are highly effective. The two plants may be considered safe for medicinal use and can be a potent source of complementary and modern medicine. Further studies are recommended on genotoxicity of this plant extracts on S. aureus and E. coli genes