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Rapid detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya

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dc.contributor.authorNgamsom, Bongkot
dc.contributor.authorWandera, Ernest Apondi
dc.contributor.authorIles, Alexander
dc.contributor.authorKimani, Racheal
dc.contributor.authorMuregi, Francis
dc.contributor.authorGitaka, Jesse
dc.contributor.authorPamme, Nicole
dc.date.accessioned2024-08-16T11:56:19Z
dc.date.available2024-08-16T11:56:19Z
dc.date.issued2019-10-10
dc.description.abstractWe report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminescence assay. Up to 80% GBS cells were isolated from spiked artificial urine samples with linear responses of bioluminescence signals from isolated cells at 2.3 × 102–9.1 × 105 CFU mL−1, demonstrating great promise for point-of-care detection of pathogenic bacteria in screening urine samples from pregnant women. Practical challenges during initial testing of the developed protocol with urine samples in Kenya are also described.
dc.identifier.urihttps://doi.org/10.1039/C9AN01808E
dc.identifier.urihttps://erepository.mku.ac.ke/handle/123456789/6177
dc.language.isoen
dc.publisherAnalyst
dc.titleRapid detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya
dc.typeArticle
dspace.entity.typePublication
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Department of Epidemiology and Biostatistics