Publication: Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection
| dc.contributor.author | Jesse, Gitaka | |
| dc.contributor.author | Njokah, Muturi | |
| dc.contributor.author | Kamita, Moses | |
| dc.contributor.author | Maina, Naomi | |
| dc.contributor.author | Kyama, Cleophas Mutinda | |
| dc.contributor.author | Sagoe, Kate Obaaya | |
| dc.contributor.author | Kinyua, Dickson Mwenda | |
| dc.contributor.author | Ndegwa, Davies | |
| dc.contributor.author | Wandera, Ernest Apondi | |
| dc.contributor.author | Thiong’o, Kelvin | |
| dc.date.accessioned | 2024-06-07T07:56:28Z | |
| dc.date.available | 2024-06-07T07:56:28Z | |
| dc.date.issued | 2023-05-07 | |
| dc.description.abstract | Globally, the emergence of the coronavirus disease (COVID-19) has had a significant impact on life. The need for ongoing SARS-CoV-2 screening employing inexpensive and quick diagnostic approaches is undeniable, given the ongoing pandemic and variations in vaccine administration in resource-constrained regions. This study presents results as proof of concept to use hybridization chain reaction (HCR) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a complex for detecting SARS-CoV-2. HCR hairpin probes were designed using the NUPACK web-based program and further used to amplify the SARS-CoV-2 N gene in archived nasopharyngeal samples. The results were visualized using agarose gels and CRISPR Cas12a-based lateral flow strips. The assay was evaluated using the gold standard, real-time polymerase chain reaction (RT-PCR), as recommended by the World Health Organization (WHO). The results show the comparative efficiency of HCR to RT-PCR. This study shows that HCR and CRISPR are viable alternatives for diagnosing SARS-CoV-2 in samples. | |
| dc.description.sponsorship | The research was funded by the African Union through the Pan African University Institute for Basic Sciences, Technology, and Innovation (PAUSTI) and the National Research Fund, Kenya. | |
| dc.identifier.citation | MDPI and ACS Style Sagoe, K.O.; Kyama, M.C.; Maina, N.; Kamita, M.; Njokah, M.; Thiong’o, K.; Kanoi, B.N.; Wandera, E.A.; Ndegwa, D.; Kinyua, D.M.; et al. Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection. Diagnostics 2023, 13, 1644. https://doi.org/10.3390/diagnostics13091644 AMA Style Sagoe KO, Kyama MC, Maina N, Kamita M, Njokah M, Thiong’o K, Kanoi BN, Wandera EA, Ndegwa D, Kinyua DM, et al. Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection. Diagnostics. 2023; 13(9):1644. https://doi.org/10.3390/diagnostics13091644 Chicago/Turabian Style Sagoe, Kate Obaayaa, Mutinda Cleophas Kyama, Naomi Maina, Moses Kamita, Muturi Njokah, Kelvin Thiong’o, Bernard N. Kanoi, Ernest Apondi Wandera, Davies Ndegwa, Dickson Mwenda Kinyua, and et al. 2023. "Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection" Diagnostics 13, no. 9: 1644. https://doi.org/10.3390/diagnostics13091644 | |
| dc.identifier.issn | 2075-4418 | |
| dc.identifier.uri | https://erepository.mku.ac.ke/handle/123456789/5850 | |
| dc.language.iso | en | |
| dc.publisher | Diagnostics | |
| dc.subject | hybridization chain reaction (HCR) | |
| dc.subject | severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) | |
| dc.subject | clustered regularly interspaced short palindromic repeats (CRISPR) | |
| dc.subject | COVID-19 | |
| dc.subject | point-of-care (POC) | |
| dc.subject | diagnostics | |
| dc.title | Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection | |
| dc.type | Article | |
| dspace.entity.type | Publication |