Publication:

Antimicrobial activity of the phytexponent against methicilin resistant staphylococcus aureus

Improper use and misuse of available antibiotics has led to emergence of many bacteria strains that are resistant to many antibiotics previously used against them. Very few antibiotics are remaining and only 50 antibiotics are currently in the pipeline. This has risen the concern as the infections caused by these resistance bacteria are a threat to humanity. Majority of these bacteria have been studied and even placed under the WHO priority list as bacteria that need urgent alternative antimicrobial agents. Among the 50 antibiotics currently in pipeline, only 32target the WHO priority pathogens.Traditionally, plants have been valuable source of new antibiotic and in the current treatment of antibiotic resistance, plant extracts containing a vast array of structurally diverse compounds acting in synergy in various pathways, this may hold a greater promising opportunity in fighting antimicrobial resistance. In present study the antimicrobial activity of the phytexponent at both low and high concentrations against methicillin resistant Staphylococcus aureus was evaluated. The diffusion technique using filter paper discs was used. The bacteria strain was sub-cultured on nutrient agar culture media and later on the inoculant prepared in sterile normal saline. At low concentrations of 100 %, 50 %, 25 %, 12.5 %, 6.25 % and 3.125 % of phytexponent was analyzed while at high concentrations, 100% of phytexponent was added in increasing volumes of10 µl, 20 µl, 30 µl, 40 µl and 50 µl . Ciprofloxacin at 0.1 ug/ml was used as standard antibiotic. The results showed that the phytexponet at high concentration greatly inhibited the growth of methicillin resistant Staphylococous aureus with zones of inhibition ranging between 10.66±0.33 mm and 26.33±0.33 while at low concentration of phytexponent ,the zones of inhibition ranged between 8.333±0.33 and 16.33±0.33 mm. Ciprofloxacin recorded zone of inhibition of 34.00±0.578 mm. In conclusion phytexponent at high concentration is effective against resistant bacteria strains such as methicillin resistant Staphylococous aureus.