Publication: Improving gonorrhoea molecular diagnostics: Genome mining-based identification of identical multi-repeat sequences (IMRS) in Neisseria gonorrhoeae
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2024-03-06
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Heliyon 10
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Abstract
Background: Curable sexually transmitted infections (STIs), such as Neisseria gonorrhoeae
(N. gonorrhoeae), are a major cause of poor pregnancy outcomes. The infection is often asymp-
tomatic in pregnant women, and a syndrome-based approach of testing leads to a missed diag-
nosis. Culture followed by microscopy is inadequate and time-consuming. The gold standard
nucleic acid amplification tests require advanced infrastructure settings, whereas point-of-care
tests are limited to immunoassays with sensitivities and specificities insufficient to accurately
diagnose asymptomatic cases. This necessitates the development and validation of assays that are
fit for purpose.
Methods: We identified new diagnostic target biomarker regions for N. gonorrhoeae using an al-
gorithm for genome mining of identical multi-repeat sequences (IMRS). These were then devel-
oped as DNA amplification primers to design better diagnostic assays. To test the primer pair,
genomic DNA was 10-fold serially diluted (100 pg/μL to 1 × 10