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Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae

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dc.contributor.authorPamme, Nicole
dc.contributor.authorRodriguez-Mateos, Pablo
dc.contributor.authorNgamsom, Bongkot
dc.contributor.authorAmeyo, Daglus
dc.contributor.authorWakaba, Patrick
dc.contributor.authorShiluli, Clement
dc.contributor.authorIles,Alexander
dc.contributor.authorGitaka, Jesse
dc.date.accessioned2024-06-07T07:33:40Z
dc.date.available2024-06-07T07:33:40Z
dc.date.issued2023-05-18
dc.description.abstractGonorrhea is the second most common sexually transmitted infection (STI) with around 87 million cases worldwide estimated in 2016 by the World Health Organization. With over half of the cases being asymptomatic, potential life-threatening complications and increasing numbers of drug-resistant strains, routine monitoring of prevalence and incidence of infections are key preventive measures. Whilst gold standard qPCR tests have excellent accuracy, they are neither affordable nor accessible in low-resource settings. In this study, we developed a lab-on-a-chip platform based on microscale immiscible filtration to extract, concentrate and purify Neisseria gonorrhoeae DNA with an integrated detection assay based on colorimetric isothermal amplification. The platform was capable of detecting as low as 500 copies/mL from spiked synthetic urine and showed no cross-reactivity when challenged with DNAs from other common STIs. The credit card–size device allows DNA extraction and purification without power or centrifuges, and the detection reaction only needs a low-tech block heater, providing a straightforward and visual positive/negative result within 1 h. These advantages offer great potential for accurate, affordable and accessible monitoring of gonorrhea infection in resource-poor settings.
dc.description.sponsorshipOpen access funding provided by Stockholm University. This work was partially funded by the University of Hull’s Quality Related Global Challenges Research Fund (QR GCRF) allocation. The authors also acknowledge Stockholm University for supporting this work through the start-up grant awarded to NP and for providing Open Access funding.
dc.identifier.citationTY - JOUR AU - Rodriguez-Mateos, Pablo AU - Ngamsom, Bongkot AU - Ameyo, Daglus AU - Wakaba, Patrick AU - Shiluli, Clement AU - Iles, Alexander AU - Gitaka, Jesse AU - Pamme, Nicole PY - 2023 DA - 2023/09/01 TI - Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae JO - Analytical and Bioanalytical Chemistry SP - 5129 EP - 5137 VL - 415 IS - 21 AB - Gonorrhea is the second most common sexually transmitted infection (STI) with around 87 million cases worldwide estimated in 2016 by the World Health Organization. With over half of the cases being asymptomatic, potential life-threatening complications and increasing numbers of drug-resistant strains, routine monitoring of prevalence and incidence of infections are key preventive measures. Whilst gold standard qPCR tests have excellent accuracy, they are neither affordable nor accessible in low-resource settings. In this study, we developed a lab-on-a-chip platform based on microscale immiscible filtration to extract, concentrate and purify Neisseria gonorrhoeae DNA with an integrated detection assay based on colorimetric isothermal amplification. The platform was capable of detecting as low as 500 copies/mL from spiked synthetic urine and showed no cross-reactivity when challenged with DNAs from other common STIs. The credit card–size device allows DNA extraction and purification without power or centrifuges, and the detection reaction only needs a low-tech block heater, providing a straightforward and visual positive/negative result within 1 h. These advantages offer great potential for accurate, affordable and accessible monitoring of gonorrhea infection in resource-poor settings. SN - 1618-2650 UR - https://doi.org/10.1007/s00216-023-04734-3 DO - 10.1007/s00216-023-04734-3 ID - Rodriguez-Mateos2023 ER -
dc.identifier.urihttps://doi.org/10.1007/s00216-023-04734-3
dc.identifier.urihttps://erepository.mku.ac.ke/handle/123456789/5849
dc.language.isoen
dc.publisherSPRINGER LINK
dc.subjectImmiscible filtration
dc.subjectDNA extraction
dc.subjectMagnetic particle
dc.subjectNeisseria gonorrhoeae
dc.subjectLAMP
dc.subjectNAAT
dc.titleIntegrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae
dc.typeArticle
dspace.entity.typePublication
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