Kyembeni, Celestine Kanini2024-10-252024-10-252023-05https://erepository.mku.ac.ke/handle/123456789/7035Multidrug drug resistance world is a predominant drift in microbiology domain and thus desires to be addressed with innovative drugs or substitute approaches of management. Traditional medicine in which plants plays a key role remains a challenge in today’s world due to lack of proper validation and documentation mechanism. Plants typically are known to contain concoctions of phytochemicals, tributary metabolites that work independently, additively, or in synergy to better the health of individuals. The study area, Kitui County- Kenya, traditional healers have been using Jatropha Curcas leaves and stem bark plant extracts as a multidrug extract. Chemicals ingredients, biological analysis and concoction effects of Jatropha Curcas crude extracts remains a research gap in the study area. The efficacies of the Jatropha Curcas herbal preparation have little or no validation at all neither is it documented. This experimental study was aimed at determining the phytochemicals compounds and antimicrobial efficacy of Jatropha curcas extracts on Escherichia coli clinical isolates from diabetic females identified with urinary tract infection in which establishment of the phytochemicals present in Jatropha curcas crude plant leaves and stem bark extracts, singly and as a concoction was done and antimicrobial efficacy of Jatropha curcas leaves and stem bark extracts, singly and as a concoction, on Escherichia coli clinical isolates of diabetic females identified with urinary tract infection. The Escherichia coli clinical isolates was obtained from diabetic females identified with urinary tract infection at Kitui County referrals Hospital and transported to Mount Kenya University, Sufficient amount of freshly mature crude Jatropha curcas leaves and stem bark were harvested from Kitui County. It is at East African Herbarium in the National Museums of Kenya where Voucher specimen’s analysis on morphological characteristics took place and matched with the others documented in the East Africa Herbarium. Phytochemistry analysis took place at East African Herbarium in the National Museums of Kenya in form of ethyl acetate, acetone, aqueous and methanol solutions for the compounds Saponins, Alkaloids, Tannins, Flavonoids, Phenol, Sterol, Terpenoids, Coumarins and Glycosides in which the profiles indicated different extraction solvents do have different effects on the phytochemical composition. After, they were subjected to clinical isolates of Escherichia coli of diabetic females identified with urinary tract infection from kitui county referrals hospital for Antimicrobial Efficacy by evaluating their Minimum Inhibition Concentration and Minimum Bactericidal Concentration where failure to proceed to Minimum Bactericidal Concentration from Minimum Inhibition Concentration was a Static indication of Jatropha curcas extracts to E.coli organism. Incubation of Culture media known to have no inoculation to check the sterility was done, known Escherichia coli standard ATCC 29218 was used as a control, a well-known standard Positive and Negative control drug was used in support of control measures. Minitab version 17.0 statistical software, ONE WAY, and TWO WAY ANOVA was used to analyze the data. Ethical endorsement was sought from Kitui County Hospital Research team management, NACOSTI, and consent was sought from the study populations.entraditional healersdrugresistanceEscherichia coliThesis