Abdi, Amina Mohamed2022-06-222022-06-222021-08http://erepository.mku.ac.ke/handle/123456789/5707Medicinal plants have gained favor among many researchers globally. This has been attributed to their therapeutic properties that has been key in management of many diseases including microbial infections, inflammation and neurodegenerative diseases. This pharmacological properties are as result of the bioactive compounds such as phenolics that are widely distributed in many plants. These compounds play a great role as antioxidants that aid in quenching free radicals preventing the development of oxidative stress and its related disorders. The evaluation of these bioactive compounds and their pharmacological properties is still insufficient. This study aimed at evaluating the total phenolic, total flavonoid and radical scavenging activity of methanol fruit and root extract of Uvariodendron anisatum. Folin-Ciocalteu’s method and calorimetric aluminum chloride method were used to evaluate total phenolic content and total flavonoid content respectively. The 2.2-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay was used to evaluate the antioxidant. L-ascorbic acid, gallic acid and catechin were used as the standards for antioxidant, total phenolic content and total flavonoid content respectively. The total phenolic content and total flavonoid were calculated from the regression equation of the gallic standard and catechin standard curve and presented as mg GAE/ g of dry weight and CE /g of dry weight respectively. The methanol root and fruit extract recorded total phenolic content of 14.627±0.078 mg GAE /g and 15.013±0.047 mg GAE /g and total flavonoid content of 7.894±0.002 mg CE /g and 8.057±0.009 mg CE /g respectively. The total phenolic content of fruit extract was significantly higher from that of root extract (P<0.05). However, the total flavonoid content in both the two extracts were not significantly different from each other (P>0.05). The 2.2-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging activity results showed a dose depended activity in both the fruit and root methanol extract of Uvariodendron anisatum and L-ascorbic acid. The methanol root extract at 0.01 µg/ml,0.1 µg/ml and 1 µg/ml recorded significantly higher percentage radical scavenging activity as than both methanol fruit extract and L-ascorbic acid (p<0.05). The standard, L-ascorbic acid at 10 µg/ml, 100 µg/ml and 1000 µg/ml significantly recorded high percentage radical scavenging activity as than both methanol root and fruit extracts (p<0.05). Methanol fruit and root extract recorded IC50 of 4.680 µg/ml and 5.080 µg/ml for fruit and root respectively while L-ascorbic acid record the IC50 of 3.420 µg/ml. The methanol fruit extract showed higher antioxidant activity that correlated to its higher phenolic compound. In conclusion, this plant is a potential antioxidant agent with the ability to scavenge the free radicalsenMedicinal plantsNeurodegenerative diseasesBioactive compoundsOther