Browsing by Author "Iliya, S."

Now showing 1 - 4 of 4
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    PublicationOpen Access
    Determination of Malondialdehyde and Superoxide Dismutase in Pulmonary Tuberculosis Subjects
    (Chinese Journal of Medical Research, 2020-03) Muhammad, Y.; Iliya, S.; Sa’idu, AY.; Anka, A.; Umar, K.; Sani, Z.; Zainab, I.
    Pulmonary tuberculosis (PTB) is a dangerous bacterial infection that attacks the lungs. It has long been documented that there is an increase circulating levels of free radicals and oxidative stress markers in TB subjects. Malnutrition and deficient antioxidant capabilities further complicate the patient’s situation. The aim of this study is to determine the serum levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in pulmonary tuberculosis patients and normal volunteers at Rasheed Shekoni Teaching Hospital Dutse, Jigawa State, Nigeria. The present study consists of one hundred and thirty-four (134) subjects, Ninety (90) among which are diagnosed TB patients and forty-four (44) apparently healthy controls attending Rasheed Shekoni Teaching Hospital Dutse. Venous samples were collected from ninety consecutive, consenting PTB on drugs and those that have not started medications. MDA levels were analysed using the method of Nadigar et al (1986). The mean values of MDA in the 24 tuberculosis infected males who are yet to commence drug was 4.0±0.32, 2.8±0.53 in those that are on drugs and 2.0±0.23 in the male control subjects. It was 4.1±0.35, 2.9±0.29 and 1.9±0.32 in female that are TB infected and yet to commence drugs, those on drugs and the control subjects respectively. The total mean MDA value for those on tuberculosis drugs was 4.0±0.33, 2.8±0.46 for those that are yet to commence the treatment and 2.0±0.28 for the control subjects, the serum levels of SOD was found significantly lower (p<0.005) in PTB subjects on treatment compared to those without treatment and control subjects. Conclusively, the findings of the current study showed that pulmonary tuberculosis patients are predisposed to oxidative stress leading to an increased MDA and consequent decreased SOD levels as compared to the control subjects.
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    PublicationOpen Access
    Ex vivo Determination of Prostate Specific Antigen (PSA) Using Green Synthesized Magnetic Iron-Oxide Nanoparticles (IONPS)
    (International Journal of Research and Scientific Innovation, 2020-04) Muhd, BK.; Umar, AA.; Muhammad, Y.; Kani, YA.; Iliya, S.; Wali, U.; Tahiru, A.; Abubakar, UF.
    The discovery and introduction of prostate-specific antigen (PSA) testing into clinical medicine enable the diagnosis, treatment and monitoring of prostatic carcinoma, and further revolutionized the management of patients with prostate cancer. Among the current developments in molecular detection of PSA is ELISA. The current research aimed at synthesizing the magnetic Iron-oxide nanoparticles for detection (assay) of prostate specific antigen and comparing the results obtained with that of the ELISA technique. It provides new advancements in molecular detections of PSA using magnetic iron-oxide nanoparticles. The magnetites were green synthesized using blackberry leaves extract, FeCl2.4H2O and FeCl3.6H2O. Serum samples (n=30) were treated and subjected to both ELISA and magnetic iron-oxide nanoparticles immunoassay techniques. Descriptive statistical analysis (α=.05) of T-test, chi-square and ANOVA using SPSS software package, reveals (p<0.005) no significance difference between values generated from ELISA and magnetic iron-oxide nanoparticles techniques. Thus, the null hypothesis which asserts that, there is no significant difference between ELISA and magnetic iron-oxide nanoparticles in PSA assay fails to be rejected. It is therefore recommended that magnetic iron-oxide nanoparticles be further exploited with regards to ex vivo determination of PSA and other tumor markers.
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    PublicationOpen Access
    Molecular Docking, Drug-Likeness and ADMET Analysis of Potential Inhibitors (Ligands) from Carica papaya Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
    (Saudi Journal of Medicine, 2020-05-17) Muhammad, Y.; Shehu, Z.; Iliya, S.; Muhd, BK.; Kani, YA.; Ahmad, MB.; Wali, U.; Yeldu, MH.
    The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease (Covid-19) pandemic has made it a universal health difficulty in both developed and developing countries, spreading fears, uncertainties and death. Worldwide, the disease is skyrocketing, spinning from a total of 124, 719 cases in 118 countries on March 12, 2020 to a high of 1,004,336 cases in 204 countries on April 2, 2020. Out of the 1,004,336 confirmed cases, 704,570 (95%) are categorized as mild cases 37,710 (5%) serious cases 51,556 deaths while 210,500 have recovered. SARS-CoV-2 belongs to beta coronavirus; usually these viruses form a polypeptide after genome transcription that needs to be cleaved enzymatically in order to produce non-structural proteins, this process is only possible with synergistic functions of Main protease or 3-chymotrypsin-like protease (3CLpro) and Papain-like protease (PLpro). Inhibiting these proteases will in no doubt interfere with replication and subsequent release of viral progenies. The current in silico study was performed to predict the possibility of 7 phytochemicals from Carica papaya as potential inhibitors of Covid-19 proteases and RNA dependent RNA polymerase. The protein structures and ligands were downloaded from RCSB Protein data bank (pdb) and PubChem respectively. The qualitative assessment of physicochemical properties, liphopilicity, water solubility, medicinal chemistry, drug likeness and ADMET were predicted virtually using swissadme. Molecular docking analysis showed that kaempferol, quercetin have good binding affinity and hydrophobic interactions with both Covid-19 proteases and RdRp. However, this hypothesis would be used as a background which needs to be proven otherwise in vitro
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    PublicationOpen Access
    Molecular studies on extra-pulmonary tuberculosis among patients attending Aminu Kano Teaching Hospital, Kano, Kano State, Nigeria
    (Dutse Journal of Pure and Applied Sciences, 2022-02-02) Mohammad, A.B.; Zakari, F.; Yusuf, A.S.; Sani, N.M.; Gwarzo, A.M.; Iliya, S.; Gwarzo, F.S.; Mukhtar, M.D.
    In 2010 World Health Organization reported that, one third of the World population had tuberculosis, and it is among the infectious disease with significant morbidity and mortality. Mycobacterium tuberculosis is a successful pathogen that has evolved several mechanisms to manipulate the host immune response. The purpose of this study was to determine the incidence of extra-pulmonary TB in patient that visited DOTs clinic, Aminu Kano Teaching Hospital, Kano Nigeria for treatment. A prospective cross-sectional laboratory based study that involved the use of questionnaire and consent/assent form prior to sample collection. Extra pulmonary tuberculosis samples were tested by MTB/RIF assay using GeneXpert. Descriptive statistics was used to analyzed the data obtained and results were given in charts and tables. Out of 71 samples analyzed along with positive control (H37RV) and molecular grade water as a negative control, 3 (4.2 %) samples had extra-pulmonary TB, among which 2 (2.8 %) were from urine samples and 1 (1.4 %) from acetic fluid. This study shows the existence of extra-pulmonary tuberculosis in the study area; and the tuberculosis was sensitive Mycobacterium tuberculosis. It is therefore important to make screening of patients with sign and symptoms of extra pulmonary tuberculosis using Xpert MTB/RIF assay as regular method for extra pulmonary tuberculosis diagnosis. This will be achieved through enlightenment of the clinicians in our health facilities, our States and local governments should strengthen the laboratory capacity for diagnosis and make the services available and reachable to the patients who need them.