Publication: Ex vivo Determination of Prostate Specific Antigen (PSA) Using Green Synthesized Magnetic Iron-Oxide Nanoparticles (IONPS)
Total Views 0
total viewsTotal Downloads 3
total downloadsDate
2020-04
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Research and Scientific Innovation
Cite this Item
Abstract
The discovery and introduction of prostate-specific
antigen (PSA) testing into clinical medicine enable the diagnosis,
treatment and monitoring of prostatic carcinoma, and further
revolutionized the management of patients with prostate cancer.
Among the current developments in molecular detection of PSA
is ELISA. The current research aimed at synthesizing the
magnetic Iron-oxide nanoparticles for detection (assay) of
prostate specific antigen and comparing the results obtained with
that of the ELISA technique. It provides new advancements in
molecular detections of PSA using magnetic iron-oxide
nanoparticles. The magnetites were green synthesized using
blackberry leaves extract, FeCl2.4H2O and FeCl3.6H2O. Serum
samples (n=30) were treated and subjected to both ELISA and
magnetic iron-oxide nanoparticles immunoassay techniques.
Descriptive statistical analysis (α=.05) of T-test, chi-square and
ANOVA using SPSS software package, reveals (p<0.005) no
significance difference between values generated from ELISA
and magnetic iron-oxide nanoparticles techniques. Thus, the null
hypothesis which asserts that, there is no significant difference
between ELISA and magnetic iron-oxide nanoparticles in PSA
assay fails to be rejected. It is therefore recommended that
magnetic iron-oxide nanoparticles be further exploited with
regards to ex vivo determination of PSA and other tumor
markers.