Browsing by Author "Kani, YA."

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    PublicationOpen Access
    Ex vivo Determination of Prostate Specific Antigen (PSA) Using Green Synthesized Magnetic Iron-Oxide Nanoparticles (IONPS)
    (International Journal of Research and Scientific Innovation, 2020-04) Muhd, BK.; Umar, AA.; Muhammad, Y.; Kani, YA.; Iliya, S.; Wali, U.; Tahiru, A.; Abubakar, UF.
    The discovery and introduction of prostate-specific antigen (PSA) testing into clinical medicine enable the diagnosis, treatment and monitoring of prostatic carcinoma, and further revolutionized the management of patients with prostate cancer. Among the current developments in molecular detection of PSA is ELISA. The current research aimed at synthesizing the magnetic Iron-oxide nanoparticles for detection (assay) of prostate specific antigen and comparing the results obtained with that of the ELISA technique. It provides new advancements in molecular detections of PSA using magnetic iron-oxide nanoparticles. The magnetites were green synthesized using blackberry leaves extract, FeCl2.4H2O and FeCl3.6H2O. Serum samples (n=30) were treated and subjected to both ELISA and magnetic iron-oxide nanoparticles immunoassay techniques. Descriptive statistical analysis (α=.05) of T-test, chi-square and ANOVA using SPSS software package, reveals (p<0.005) no significance difference between values generated from ELISA and magnetic iron-oxide nanoparticles techniques. Thus, the null hypothesis which asserts that, there is no significant difference between ELISA and magnetic iron-oxide nanoparticles in PSA assay fails to be rejected. It is therefore recommended that magnetic iron-oxide nanoparticles be further exploited with regards to ex vivo determination of PSA and other tumor markers.
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    PublicationOpen Access
    Molecular Docking, Drug-Likeness and ADMET Analysis of Potential Inhibitors (Ligands) from Carica papaya Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
    (Saudi Journal of Medicine, 2020-05-17) Muhammad, Y.; Shehu, Z.; Iliya, S.; Muhd, BK.; Kani, YA.; Ahmad, MB.; Wali, U.; Yeldu, MH.
    The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease (Covid-19) pandemic has made it a universal health difficulty in both developed and developing countries, spreading fears, uncertainties and death. Worldwide, the disease is skyrocketing, spinning from a total of 124, 719 cases in 118 countries on March 12, 2020 to a high of 1,004,336 cases in 204 countries on April 2, 2020. Out of the 1,004,336 confirmed cases, 704,570 (95%) are categorized as mild cases 37,710 (5%) serious cases 51,556 deaths while 210,500 have recovered. SARS-CoV-2 belongs to beta coronavirus; usually these viruses form a polypeptide after genome transcription that needs to be cleaved enzymatically in order to produce non-structural proteins, this process is only possible with synergistic functions of Main protease or 3-chymotrypsin-like protease (3CLpro) and Papain-like protease (PLpro). Inhibiting these proteases will in no doubt interfere with replication and subsequent release of viral progenies. The current in silico study was performed to predict the possibility of 7 phytochemicals from Carica papaya as potential inhibitors of Covid-19 proteases and RNA dependent RNA polymerase. The protein structures and ligands were downloaded from RCSB Protein data bank (pdb) and PubChem respectively. The qualitative assessment of physicochemical properties, liphopilicity, water solubility, medicinal chemistry, drug likeness and ADMET were predicted virtually using swissadme. Molecular docking analysis showed that kaempferol, quercetin have good binding affinity and hydrophobic interactions with both Covid-19 proteases and RdRp. However, this hypothesis would be used as a background which needs to be proven otherwise in vitro